Journal: bioRxiv

Article Title: ECgo: All-Optical Induction of Single Endothelial Cell Injury and Capillary Occlusion in the Brain

doi: 10.1101/2025.05.27.656398

Figure Lengend Snippet: (A) Quantification of patent capillaries subjected to irradiation in the presence of Ps2P , either pretreated with anticoagulants to prevent occlusion or post-treated with rtPA to promote recanalization. All treatments failed to prevent blood flow cessation or to restore blood flow, respectively. (B) Reduction in blood flow upon capillary irradiation in the presence of anticoagulant drugs (left): Aspirin (N = 2, n = 10), heparin (N = 3, n = 17), clopidogrel (N = 4, n = 12) or blood flow recovery following reperfusion therapy with rtPA (N = 2, n = 18; right). Points represent single capillaries. (C) Representative two-photon images showing stalled blood cells inside the targeted capillary (left: leukocyte, middle: red blood cells (RBCs), right: platelets). Channels are displayed separately, with the merged image in the top left. Scale bars: 10 μm. (D) Representative maximum intensity two-photon 30-µm-z-projections of a Claudin5-EGFP reporter mouse. White arrowheads indicate individual EC nuclei. Scale bar: 10 μm. (E) Representative two-photon images of an EC injured capillary, showing pre-irradiation EC morphology (left) and post-irradiation damage (right). Line scan kymographs (cyan rectangles) using third-harmonic generation (THG) imaging confirm the presence and absence of blood flow pre- and post-irradiation, respectively. White arrowhead points towards injured EC nuclei. Scale bars: 10 μm. N = number of animals, n = number of capillaries.

Article Snippet: Clopidogrel (Tocris, Cat.# 1820) administration included either a 4-day treatment at a lower dose (20 mg/kg; one i.p. injection per day over 4 d) or a higher single dose injection (100 mg/kg, i.p.) on the capillary irradiation day, with a minimum 3-h waiting period between injection and capillary irradiation.

Techniques: Irradiation, Imaging

Journal: Journal of Inflammation Research

Article Title: Platelet Hyperactivation Plays a Critical Role in Exacerbating Skin Lesions in Rats with Psoriasis and Blood Stasis Syndrome

doi: 10.2147/JIR.S560634

Figure Lengend Snippet: Clopidogrel alleviates skin lesions in rats with psoriasis and BSS. ( A ) Dorsal skin appearance and Subcutaneous blood vessel morphology in rats. ( B ) PASI score. ( C and D ) Skin HE staining. ( E – G ) Relative mRNA levels of CCL20, S100A8 and S100A9 in lesions. ( H and I ) Neutrophil and lymphocyte proportions (CBC). *P < 0.05, **P < 0.01, ****P < 0.0001 versus Control. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 versus IMQ+BSS.

Article Snippet: Clopidogrel (Cat: HJ20171237) was procured from Sanofi (Hangzhou) Pharmaceuticals Co., Ltd. (Hangzhou, Zhejiang Province, China).

Techniques: Staining, Control

Journal: Journal of Inflammation Research

Article Title: Platelet Hyperactivation Plays a Critical Role in Exacerbating Skin Lesions in Rats with Psoriasis and Blood Stasis Syndrome

doi: 10.2147/JIR.S560634

Figure Lengend Snippet: Clopidogrel alleviates Blood stasis manifestations in rats with psoriasis and BSS. ( A ) Whole blood flow resistance analysis. ( B ) ROI values for circulatory perfusion in ears, leg, and tail. ( C ) Spleen and blood vessel HE staining. ( D ) Thickness of vessel wall. ( E and F ) IHC detection of VEGFA expression in rat skin. ( G and H ) IHC detection of CD34 expression in rat skin. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control. # P < 0.05 versus IMQ+BSS.

Article Snippet: Clopidogrel (Cat: HJ20171237) was procured from Sanofi (Hangzhou) Pharmaceuticals Co., Ltd. (Hangzhou, Zhejiang Province, China).

Techniques: Staining, Expressing, Control

Journal: Journal of Inflammation Research

Article Title: Platelet Hyperactivation Plays a Critical Role in Exacerbating Skin Lesions in Rats with Psoriasis and Blood Stasis Syndrome

doi: 10.2147/JIR.S560634

Figure Lengend Snippet: Network pharmacology and molecular docking identify P2ry12 and GPIIb/IIIa as a potential target of clopidogrel for psoriasis with BSS. ( A ) Psoriasis-Clopidogrel target intersection. ( B ) PAF-Clopidogrel target intersection. ( C ) PPI network of Clopidogrel-Psoriasis shared targets. ( D ) PPI network of Clopidogrel-PAF shared targets. ( E ) Core targets screened from Clopidogrel-Psoriasis. ( F ) Core targets screened from Clopidogrel-PAF. ( G ) GO enrichment for Clopidogrel-Psoriasis targets. ( H ). GO enrichment for Clopidogrel-PAF targets. ( I ) KEGG enrichment for Clopidogrel-Psoriasis targets. ( J ) KEGG enrichment for Clopidogrel-PAF targets. ( K and L ) Clopidogrel-core target docking scores. ( M – P ) Molecular docking schematics for P2ry12/PTGS1/GPIIIa/SRC with Clopidogrel.

Article Snippet: Clopidogrel (Cat: HJ20171237) was procured from Sanofi (Hangzhou) Pharmaceuticals Co., Ltd. (Hangzhou, Zhejiang Province, China).

Techniques:

Journal: Journal of Inflammation Research

Article Title: Platelet Hyperactivation Plays a Critical Role in Exacerbating Skin Lesions in Rats with Psoriasis and Blood Stasis Syndrome

doi: 10.2147/JIR.S560634

Figure Lengend Snippet: Clopidogrel reduces platelet activation and aggregation in rats with psoriasis and BSS. ( A – F ) Relative mRNA levels of itgb3, itga2b, P2Y12, GPIba, GP5, GP9, in lesions. ( G and H ) Relative mRNA levels of Selp, and PAFR in lesions. ( I and J ) Flow cytometric detection of CD62P. ( K and L ) IHC detection of PAFR expression in rat skin. *P < 0.05, **P < 0.01, ****P < 0.0001 versus Control. # P < 0.05 versus IMQ+BSS.

Article Snippet: Clopidogrel (Cat: HJ20171237) was procured from Sanofi (Hangzhou) Pharmaceuticals Co., Ltd. (Hangzhou, Zhejiang Province, China).

Techniques: Activation Assay, Expressing, Control